SOLVED: In anion exchange chromatographySelect one:a. the column contains negatively charged beads where positively charged proteins bindb. the column contains positively charged beads where negatively charged proteins bindc.the column contains both positive and negatively charged beads where proteins bind depending on their net charge
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SOLVED: Three proteins with an overall charges of 0, -5, and -8 are passed through an anion exchange column equilibrated in a buffer with low ionic strength and eluted with an increasing gradient of salt in a buffer. Which protein will elute last from the column?1. Protein with no charge.2. Protein with eight negative charges.3. Protein with five negative charges.4. Both charged proteins will elute at the same time.
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