Finally, we will verify the expression levels of DEGs through quantitative real-time polymerase chain reaction (qRT ... The transcriptome library preparation used the DNBSEQ-T7 platform (MGI Tech).

This manuscript characterizes a mutated clone of RNA polymerase I in yeast, referred to as SuperPol, to understand the mechanisms of RNA polymerase I elongation and termination. The authors present ...

When RNA molecules are synthesized by cells — a critical process in the creation of proteins and other cellular functions — they typically undergo a series of “folding” events that determine their ...

With over 750 million long RNA reads across 14 human cell lines, the Singapore Nanopore Expression (SG-NEx) dataset is designed to help researchers decode RNA complexity with greater precision ...

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The study characterises an RNA polymerase (Pol) I mutant (RPA135-F301S) named SuperPol. This mutant was previously shown to increase yeast ribosomal RNA (rRNA) production by Transcription Run-On (TRO) ...

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Besides their tasks in vivo, DNA polymerases are the workhorses in numerous biotechnology applications such as the polymerase chain reaction (PCR), cDNA cloning, genome sequencing, nucleic acids-based ...