Here we optimize an Epi-editing platform based on mRNA delivery of dCas9-KRAB/DNMT3AL silencers (CRISPRi) and dCas9-2XVP64 activators (CRISPRa), resulting in transient editor expression suitable for ...

We used the protein hashes and the dCas9 cDNA (the presence or absence of the KRAB domain) to demultiplex and determine the cell line—CRISPRi or CRISPRa—cells containing a single sgRNA per cell were ...

CRISPRi is based on the CRISPR-Cas genome editing system but lacks the ability to cut DNA, a so-called "dead CAS" (dCAS) and is fused to a KRAB repressor domain. Using a short RNA as a molecular ...

One of these molecular biological tools is CRISPRi (from "CRISPR interference"). CRISPRi blocks genes and gene expression without modifying the DNA sequence. As with the CRISPR-Cas system also ...

One of these tools is CRISPRi (CRISPR interference) which blocks genes and gene expression without modifying the DNA sequence. Like the traditional CRISPR mechanism, the guide RNA directs a ...

In contrast to gene editing approaches, dCas9-KRAB (Krüppel associated box domain)–mediated CRISPRi is generally reversible and titratable, and circumvents the risks associated with direct ...

CRISPR activating (CRISPRa) and CRISPR interference (CRISPRi) screens were performed in the LNCaP PC cell line stably expressing either dCas9-VP64 or dCas9-KRAB, respectively. Cells were transduced in ...