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An important step in the production of CAR-T cells is the evaluation of CAR expression. Usually, this is performed by flow cytometry using target antigens, protein L, or anti-Fab antibodies as ...

After the cells were incubated with doxycycline (−) or (+) medium for 48 h, expression levels of p53-associated or -downstream proteins were compared. Stably transfected cells showed induced p53 ...

As cell therapies are increasingly integrated into conventional cancer treatment strategies, attention has turned to addressing challenges in production and quality control that hinder cell therapy ...